PI: Dr Thomas Gorochowski
Co-I: Jeff Nivala (University of Washington)
This project will combine a mixture of protein design, nanopore sequencing, and genome engineering to build a new generation of yeast strains for supporting the rational optimisation of bioprocesses. It will build on advances in multiplexed CRISPR-based genome editing and employ Nanopore-addressable protein Tags Engineered as Reporters (NTERs) to monitor gene expression levels using nanopore sequencing. The project is a collaboration between the Biocompute Lab based in the School of Biological Sciences at the University of Bristol, and the Nivala Lab at the Molecular Engineering & Science Institute at the University of Washington, USA.